| 신세포암 쥐 모델에서 냉동수술 후 항종양 면역효과 |
|
여정균, 조대연, 박민구 |
|
인제대학교 의과대학 비뇨기과학교실 |
| Anti-tumor Immune Response after Cryoablation in Renal Cell Carcinoma Murine Model |
|
Jeong Kyun Yeo , Dae Yeon Cho , Min Gu Park |
|
Department of Urology, Inje University College of Medicine, Pusan, Korea |
Correspondence:
Min Gu Park
|
|
|
| This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Abstract |
|
aneous inoculation of Renca cells in the thigh. After 7 days, the tumors were removed using liquid nitrogen in cryoablation group and bipolar electrocoagulation in electrocautery group. For twelve days after re-inoculation of Renca cells at contralateral thigh, tumor volumes were measured daily to assess the effect against the growth of tumor. The immunocyte levels (T4, T8, B and NK cell) were determined to evaluate immune activity by FACS (Fluorescence activated cell sorter) analysis. The effect of cryoablation to induce apoptosis of tumor was evaluated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end-labeling) assay. Results: The tumor volume of cryoablation group was significantly smaller than that of electrocautery group and control (p＜0.05). Comparing with control, T cell level was significantly increased after cryoablation (p＜0.05), but no group had a significant difference in the levels of B cell and NK cell by FACS analysis. The apoptosis index % of cryoablation group was significantly increased than that of control group (p＜0.05) by TUNEL. Conclusions: Cryoablation could result in the inhibition of re-inoculated tumor growth and induce T cell mediated immune response. The active immune response may be attributed to the apoptosis of tumor after cryoablation. (Korean J Urol Oncol 2016;14:32-38) |
| Key Words:
Cryoablation, Renal cell carcinoma, Immunology |
|
PDF Links
Download Citation
